For total protein, the membrane was probed with primary anti-SGK1 antibody (AbSci, Vancouver, WA, Canada), anti-phosphorylated SGK1 (Ser422) antibody (AbSci, Vancouver, WA, Canada), ...
Mechanistically, ERK inhibition increases mitochondrial function and SGK1 phosphorylation via mTOR2 activation, which leads to osteoblast differentiation and production of angiogenic and osteogenic ...